a431 pervanadate  (Santa Cruz Biotechnology)

Journal: Hepatology (Baltimore, Md.)

Article Title: Derivation, characterization, and phenotypic variation of hepatic progenitor cell lines isolated from adult rats.

doi: 10.1053/jhep.2002.31355

Figure Lengend Snippet: Fig. 3. Group phenotyping of LPC strains and clonal lines cultured on STO feeder layers. Cellular extracts from 22 sources were made 3 days postplating and analyzed (lanes 4-25) for AFP, albumin, CK14, c-kit, CD34, and desmin by Western blotting. The names of individual cell strains and cell lines are shown above each lane; the larger single numbers above the names designate group assignments (see Table 2). Extracts used as internal controls were obtained from neonatal liver (BRL) and, as indicated, from bone marrow and skeletal muscle (lane 1), STO fibroblasts (lane 2), freshly isolated adult rat hepatocytes and, as annotated, human A431 cells (lane 3). The 6 positive control markers included AFP, albumin, CK14, and c-kit (BRL), CD34 (bone marrow), and desmin (skeletal muscle) in lane 1; and CK14 (A431 cells) and albumin (adult hepatocytes) in lane 3. Negative controls were provided by STO cells for all markers (lane 2), by adult rat hepatocytes for AFP and c-kit (lane 3), and for CK14 (not shown). Molecular weights of the 6 markers are listed to the right of lane 25.

Article Snippet: The following materials were obtained commercially: dexamethasone and bFGF (Sigma); Oncostatin M (R&D Systems, Minneapolis, MN); Matrigel (Collaborative Research, Bedford, MA); human epidermoid carcinoma cell line A431 lysates (Santa Cruz Biotechnology, Santa Cruz, CA).

Techniques: Cell Culture, Western Blot, Isolation, Positive Control